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Our Services

OUR SERVICES

  • Genetic Diagnosis
  • Infertility
  • Foetal Medicine
  • Medical Genetics
  • Gynecology and Obstetrics 
  • Paternity Test
  • Preimplantation Genetic Applications (PGD/PGS)
  • Prenatal Diagnosis
  • Reproductive Genetics
  • Molecular Oncology
  • Molecular Hematology
  • Pharmacogenetics
  • Genomic Tumor Advisory
  • School for Cancer
  • Cardiovascular Diseases
  • Nutrition Genetics
  • Athlete Genetics
CYTOGENETICS LABORATORY

In our center, we can diagnose chromosomal diseases. In this department, chromosomes are obtained from peripheric blood, cord blood, amniotic fluid, placental cells, skin fibroblast, gonad tissue, abort material and tumor cells.

Conventional cytogenetics gives information about all anomalies detectable at microscopic levels in whole genome and  therefore, it is accepted as the highest standard in prenatal and postnatal diagnosis.

MOLECULAR CYTOGENETICS LABORATORY

It is the department wheret we use cytogenetics and molecular methods together.

Quantitative and 5-10Mb sized structural chromosome anomalies can be detected with conventional cytogenetic methods. Submicroscopic changes smaller than 5Mb can’t be detected with conventional cytogenetics but last 15 years, performing Molecular Cytogenetics techniques like FISH and Array-CGH made it possible.

  1. FISH: It is a method that includes stages such as giving fluorescent light by enzymatic way to DNA/RNA sequences inherent in specific parts of the chromosomes and signing them with prob material, forming hybrid with the targeted DNA/zone and analysing these hybrid signals with microscope using specific filters. Aneuploidy is detected by FISH analysis – this method is also used in detecting cancer and hematological conditions.
  2. Array-CGH: A-CGH is a technique that can detect genomic irregularities at DNA level and whole genome and it is also called molecular karyotyping. This method has advantages such as the ability to detect copy number changes between 50-100 Kb without using metaphase plaque and ability to work with a limited number of DNA samples. We have Agilent Microarray Platform in our laboratory.
MOLECULER GENETIC LABORATORY

This department monitors and studies the changes at DNA and RNA levels.

Following techniques are used in this laboratory;

  1. Real-Time PCR: It is the technique to detect and quantify DNA real-time by using fluorescent dyes. Fluorescent signal increases proportionally with the amount of PCR product. We have LightCycler® 480 Instrument II system in our laboratory.
  2. MLPA; is an “easy-to-handle in laboratory” and multiplex technique that can detect even a single nucleotid change and the change of number of copies for more than 50 zones in DNA/RNA sequence.
  3. DNA Sequencing Methods:
  • Sanger Sequencing: Uses DNA polymerase, dNTP (deoxyribonucleoside triphosphate) and ddNTP (dideoxyribonucleoside triphosphate) as substrates. Technically, sequence analysis comprises of 3 steps.  In our laboratory, 3100 16 Capillary Sequencing device is used.
  1. a) PCR
  2. b) Sequence Reaction
  3. c) Gel electrophoresis and computer assessment
  • New Generation Sequencing (NGS): NGS is a high-numbered sequencing method that is based on simultaneous sequencing of millions of DNA fragments gathered from one sample. We have Ion Torrent PGM and Illumina MiSeq platforms in our lab.